Faced with fitting well over 30 experiments at room and low temperature at multiple frequencies, as all of my experiments are spin-labelled mutants of the same protein then surely their EPR properties would be similar. I decided that there must be a …
The Savitzky-Golay smoothing function essentially applies a local polynominal fitting routine to a series of data points to then find the smoothed data points. This has the benefit that for sharp intense peaks (common to EPR) that the line broadening …
DeerAnalysis is a program from the team at ETH Zurich that allows for the easy conversion of DEER / PELDOR data into distance distributions. The program itself runs within MATLAB which is renowned for being difficult to make high quality figures …
The Bruker spectrometers output data by default in the Bruker BES3T format, which whilst being perfectly usable is not recognisable by most operating as standard.
This script was originally written for the benefit of the project students in the lab …
This script allows for the writing of cw-EPR Bruker BES3T files (.DTA/.DSC) files from MATLAB onto your computer
The script uses a graphical user interface to select file name and location, so minimal user line experience is required.
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This script allows for the loading of cw-EPR Jeol files (*.esr) into the MATLAB environment for data analysis.
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When no inputs are selected a “point and click” interface guides the user through selecting a file
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Traditionally the MTSL spin label bound to a Cysteine residue (Cys) is given the residue code R1A, at least in crystallography structures.
However, some computer modelling programs prefer the identifier CYM. To switch between the two I’ve …
Determination of the effect of increasing microwave powers on the saturation behaviour of a sample can give information regarding the relaxation properties of the sample. …
Feature implemented in EPRtoolbox or download now PDBs are great, however from time to time you only actually want one protein chain to analyse at a time. What you need to do then is split the PDB of many chains into separate PDB files.
This MATLAB …
Feature implemented in EPRtoolbox or download now
MMM is a program from the team at ETH Zurich which allows for the attachment of spin labels in silico. Once attached PELDOR (aka DEER) spectra can be simulate.
With time a temperature assay can be …
Feature implemented in EPRtoolbox or download now
Carrying on from the 2 spin label temperature assay figure creation script. I found myself trying to repeat my success on a system with 3 spin labels.
Such systems give added complication as we now …
| Version | Release date | Notes |
|---|---|---|
| v14.09 | 7th September 2014 | 1500+ downloads |
| v14.05 | 15th April 2014 … |
cw-EPR spectra are common place in the lab, but often we report them simple as intensity against magnetic field in Gauss (G) or milli-Tesla (mT). However, in reality what we are actually interested is the frequency independent g-value.
The problem is …
This script has been superceded by cwViewer
cwPlotter is a tool for the easy opening, analysis, manipulation and plotting of cw EPR data.
With an intuitive user interface, users can quickly create professional looking figures in seconds.
cwPlotter …
The amino acid converter (AAconverter) is a small user interface that I’ve put together for MATLAB that will transform amino acids between the 1 letter or 3 letter representation. Useful for any amount of DNA work.
Simply, type or paste (from …
This script loads Bruker EPR files into MATLAB.
The script has been extensively tested with Bruker BES3T files (.DTA/.DSC). Originally it was built for cw-EPR files, but functionality has been added for 3 dimensional cw experiments (.DTA/.DSC/.YGF) …
Implemented in the EPRtoolbox or direct download
This script is a quick way of presenting cw-EPR spectra by taking data from arrays of magnetic field and intensity
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Where, x and y can have any number of columns (so long there is …
Feature implemented in EPRtoolbox or download now (requires fpeak)
A simple MATLAB script to open an EPR spectra (requires eprload from S. Stoll’s EasySpin), baseline corrects to zero, plots the result and then finds all of the peaks.
It …
This feature has been removed from the EPR toolbox as of version 12.9, due to MMM 2011.2 directly implementing this feature. This page remains for information purposes only. Feature implemented in EPRtoolbox
MMM by default saves calculated rotamers …
Feature implemented in EPRtoolbox or download now
cw-EPR are useful, but when comparing numerous spectra things can become quite challenging especially when different powers or sample concentrations are used.
By normalising (normalizing by American …
By